How long is d1s80

The first four repeats and the last three repeats were conserved expect for the allele The allele 21 appears to contain a nucleotide substitution in the 19th repeat. However, we do not know whether the nucleotide substitution occurred in the PCR amplification before the cloning or was derived from an individual who provided the allele.

The lengths of all the alleles in this study were calculated using the following formula reported by Sekiguchi et al. The sequencing result revealed that all the alleles in our allelic ladder marker had the correct length corresponding to the allele number, and we think that this marker can be used for more effectively typing D1S A new sequenced allelic ladder marker for D1S80 typing. Int J Legal Med — J Forensic Sci — Leg Med — Article Google Scholar.

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Correspondence to Koji Fujii. Reprints and Permissions. Fujii, K. J Hum Genet 49, — Download citation. Received : 24 October Accepted : 11 December Published : 01 March Issue Date : March Anyone you share the following link with will be able to read this content:. Sorry, a shareable link is not currently available for this article. Provided by the Springer Nature SharedIt content-sharing initiative.

Advanced search. Skip to main content Thank you for visiting nature. Download PDF. Abstract A sequenced allelic ladder marker that contains 32 alleles consisting of alleles 13—44 was developed for D1S80 MCT typing. Introduction D1S80 pMCT , GenBank accession number D is a variable number of tandem repeat locus located on chromosome 1p with a repeat unit of 16 bp Nakamura et al.

Materials and methods Blood samples were collected from 23 Japanese individuals that were heterozygous at the D1S80 locus. The most common alleles were repeat units 24 Other alleles were 25 7.

The allele with 23 repeat units was not observed. Results were in Hardy-Weinberg linkage disequilibrium. Observed heterozygosity was 0. Theta k value was 4.